Table 1.
Characteristics of 30 microsatellite loci in Cardiocrinum giganteum var. yunnanense.
| Locus | Primer sequences (5′-3′) | Repeat motif | Size range (bp) | Ta (°C) | GenBank Accession No. |
|---|---|---|---|---|---|
| CGY003 * | F: TATGGAGGGTTCTATTGCC | (AG)9 | 214–223 | 52 | JQ340036 |
| R: GGTTTCAGAGTTCATTGGAT | |||||
| CGY005 | F: AAAGGAGGGGAAGGCATAGT | (AG)5AT(AG)5 | 257 | 55 | JQ340037 |
| R: CTAAGAGACCGCCTCCTCAT | |||||
| CGY006 * | F: TGGTATTGTCAGAATCTCAT | (TG)10(AG)13 | 169–182 | 50 | JQ340038 |
| R: AGGTTGTTGGGTGGAGTAGT | |||||
| CGY007 * | F: TGTGTGAGTTTGAGCATAAT | (AG)15 | 130–145 | 52 | JQ340039 |
| R: CGGATACTCAGTGAACCTAC | |||||
| CGY011 * | F: ACAATAACCCCAGTAGACC | (AG)14 | 164–168 | 55 | JQ340040 |
| R: TGGGTGCTCATCAAGTGTC | |||||
| CGY012 * | F: CGAACTGAACATTGAGAAGA | (AG)15 | 119–127 | 50 | JQ340041 |
| R: ATTACACTCTGACAACACCG | |||||
| CGY015 | F: TCTCAAGTAAATCCAACAAAT | (AG)16 | 156 | 50 | JQ340042 |
| R: AAGGTATTGGAATGGCGAT | |||||
| CGY028 * | F: ATGAGAAAGAGAGATGAAAG | (AG)13 | 147–153 | 48 | JQ340043 |
| R: TAAAGTGTGTGTAGGTGGAG | |||||
| CGY029 * | F: TTCATTATCATCTCGGACAC | (TG)7(AG)14 | 157–165 | 48 | JQ340044 |
| R: AGAAGGTCAACCGAACACAT | |||||
| CGY031 * | F: ACTCCTCTACCCTTTCACCA | (AG)9 | 163–173 | 48 | JQ340045 |
| R: CATGATATTTATACTGAGGTTCT | |||||
| CGY035 | F: AACAAAAGAAAGCAGTAGAA | (TC)11 | 184 | 48 | JQ340046 |
| R: TATGATAGAGCAAAAGAGGG | |||||
| CGY036 * | F: TATCGCCTTCTTACACTTA | (AG)18 | 178–182 | 50 | JQ340047 |
| R: TGAGCCGATTCCTACATTTT | |||||
| CGY037 | F: TCCAAGAGAGAAAGCATCAA | (TC)9 | 144 | 50 | JQ340048 |
| R: ATGGCAGAATCACAATAAGT | |||||
| CGY043 * | F: TTTCAGCCACCCTCACTATT | (AG)8 | 180–186 | 50 | JQ340049 |
| R: CTCCTATTTTTACAAGACGC | |||||
| CGY053 * | F: TGCCAGAAAAGAATCACAA | (AG)11 | 144–152 | 50 | JQ340050 |
| R: ATGACCCTTCCTAATTCG | |||||
| CGY054 * | F: ACCCAAATAAAGTAACAGACCA | (TC)16 | 197–207 | 57 | JQ340051 |
| R: TGCCCCATCACATCCCCACC | |||||
| CGY058 * | F: GTAGTTTCCTTCATCGCCTT | (TC)15 | 243–251 | 57 | JQ340052 |
| R: CCACACAGGGAGGCATCTTT | |||||
| CGY064 * | F: TATTTCTTATTCTTCACCTC | (AG)14 | 121–127 | 47 | JQ340053 |
| R: AAAACCAATAAAATCCCTC | |||||
| CGY065 * | F: CCGTTGGGATTATGAGTATT | (TC)19 | 167–175 | 50 | JQ340054 |
| R: CAGCATAGAGCATAGCCCTT | |||||
| CGY066 * | F: TGGAGAGATTCAGGTTCATA | (AG)14 | 218–230 | 52 | JQ340055 |
| R: GAGACCATACATCACTAAATCA | |||||
| CGY067 | F: GTGACCTTAGGAGTATATTAGC | (AG)10 | 237 | 55 | JQ340056 |
| R: CGGAAATGGCTACTAACTAAGA | |||||
| CGY072 | F: AGATGAAGGAGTAGGGACAA | (TC)8 | 305 | 55 | JQ340057 |
| R: CAAACTCCCACTCACCATTC | |||||
| CGY073 * | F: GTCTCCCTCCTTCTCAAAAT | (TC)7 | 250–258 | 55 | JQ340058 |
| R: CTTCTTGCCCCCACTAACTT | |||||
| CGY075 * | F: GCCATAGAGACATAGGGAGG | (AG)19 | 213–221 | 55 | JQ340059 |
| R: ATGAAACCTGACCTAAGCAC | |||||
| CGY083 * | F: CCTACTCATTTTTCAACTTTC | (TG)13 | 290–298 | 52 | JQ340060 |
| R: GCCCATTCCCAACCACTATT | |||||
| CGY091 * | F: TGGACACATTTTTGGCTAAG | (AC)5AT(AC)11 | 120–132 | 50 | JQ340061 |
| R: CGACGATTAGGGCAAAGGTA | |||||
| CGY099 | F: TCATTCCACTCCACCATAAA | (AC)12 | 119 | 50 | JQ340062 |
| R: ATACCTAACCATCTTCCAAT | |||||
| CGY105 | F: CCCAAAAATAATCATCAAGC | (AC)7 | 152 | 52 | JQ340063 |
| R: CACCTACCCTGCTTTGTTCA | |||||
| CGY110 * | F: ATAGTGTAGCAGTGAAGCGA | (TG)7 | 115–119 | 57 | JQ340064 |
| R: TGTGGTTGGTTTCTCATTGC | |||||
| CGY111 | F: TGACACCCCCATACTTAGAC | (TG)7 | 115 | 50 | JQ340065 |
| R: TCTCATCACTCTATCTCATT |
Ta: PCR annealing temperature;
*
displayed polymorphisms.