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(A) Interaction of DWWA and CaM. Dictyostelium CaM fused with GST and bound to glutathione-Sepharose beads and extracts from cells expressing GFP-DWWA were mixed. GST-CaM-beads were washed, extracted, and subjected to SDS-PAGE. GFP-DWWA was detected using an anti-GFP antibody on Western blots. (B) Effect of W-7 on DWWA binding to CaM. GST-CaM-beads and cell extracts containing GFP-DWWA were incubated in the absence or the presence of 100 μM W-7 in the Ca2+-free buffer.
