Figure 3.

Expression of dwwA during the life cycle of Dictyostelium and subcellular localization of GFP-DWWA. (A) RT-PCR. Wild-type cells were developed on nonnutrition agar for the times indicated, after which the DNA was amplified using the same amount of total RNA as the template in each case. Amplification of IG7 served as an internal control (Chang et al., 1996). (B) Localization of the GFP-DWWA in living wild-type cells (left) and in cells fixed with 3.7% formaldehyde (right). Full-length DWWA was concentrated along the cell cortex. (C) Localization of GFP-DWWA in fixed cells during the cell cycle. Bar, 10 μm.