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. 2004 Feb;15(2):435–446. doi: 10.1091/mbc.E03-05-0329

Figure 7.

Figure 7.

Distribution of actin and myosin II in mitotic wild-type (A) and dwwA-null (B) cells. (A) Wild-type cells were transformed with plasmid carrying the GFP-myosin II gene (top). GFP-myosin II localized to cleavage furrows in mitotic wild-type cells. F-actin was visualized by fixing cells with 3.7% formaldehyde and staining with rhodamine-phalloidin (bottom). F-actin was enriched along both poles of the two daughter cells. (B) In dwwA-null cell, GFP-myosin II in a dumbbell-shaped cell was localized in the cleavage furrow (top), as in wild-type cells. Middle and right pictures in the top panel show cells at the final step of cytokinesis. Cells that proceed to the final step of cytokinesis accumulated GFP-myosin II in the thin cytoplasmic bridge connecting the two daughter cells, as well as in the tapered region of the cell body connected to the cytoplasmic bridge. Bottom, distribution of F-actin at the final step of cytokinesis with long and thin cytoplasmic bridges. Note the excessive accumulation of F-Actin around the cell periphery. Bar, 10 μm.