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Differential permeabilization of plasma and ER membranes. HeLa cells (2 × 106) were permeabilized with SLO or digitonin (Dig; 0.04 or 0.1%). The cell remnants were pelleted in a Microfuge, and S and P fractions, corresponding to 5 × 104 cells, were analyzed by SDS-PAGE and immunoblotting with the indicated antibodies.
