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The ER membrane does not constitute a significant barrier to small molecules. HeLa cells were permeabilized with 0.04% digitonin (A) or SLO (B) and incubated with 0.5 mM sulfo-NHS-LC-biotin for different periods of time. Biotinylated proteins were recovered after incubation with streptavidin beads and analyzed by SDS-PAGE and immunoblotting with antibodies to different proteins. The bands were quantitated using a Fujix PhosporIimager and the software Image Gauge 3.0.
