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. 2004 Feb;15(2):456–467. doi: 10.1091/mbc.E03-03-0179

Figure 2.

Figure 2.

Hyphal-inducing conditions induce SOD5 expression. (A) Serum, rather than temperature, induces SOD5 transcription. Northern blot analysis of the SOD5 transcripts in C. albicans strain SC5314 grown in the log phase in indicated media (YPD and YPD containing 10% serum) at indicated temperatures. (B) SOD5 transcription is up-regulated in IMDM medium. Northern blot analysis of the SOD5 transcripts in C. albicans strain SC5314 grown in the log phase in IMDM medium at 30 and 37°C. Northern blot analysis of the actin (ACT1) transcripts in C. albicans strain SC5314 was used as an internal loading control. Relative intensities are shown below each of the genes analyzed. Relative intensities of cells grown at 37°C are underlined and quantified independently from SOD5 intensities obtained at 30°C. Quantifications were performed by NIH Image software (RSB-NIMH-NIH, http://rsb.info.nih.gov/nih-image/).