Table 2.

Plasmids and oligos used in this study

A. Plasmids	Description	Source
pVEC	CaARS CaURA3	Magee and Magee (1997)
p37	CaARS upstream flank 1kb CaSOD5 downstream flank 1kb	This study
p37H	CaARS upstream flank 1kb CaHIS1 downstream flank 1kb	This study
p37U	CaARS upstream flank 1kb hisG-CaURA3-hisG downstream flank 1 kb	This study
pSOD5-110	CaARS CaURA3 upstream flank 1kb CaSOD5 downstream flank 1kb	This study
p5921	hisG-CaURA3-hisG	Fonzi and Irwin (1993)
pRM100	CaARS CaHIS1	Pla et al. (1995)

B. Oligos	Sequence	Used for
ODH157(EcoRI)	GGAATTCAAACCTGGTCTACCAGAAGTT	SOD5 + 1kb flank F
ODH158 (EcoRI)	GGAATTCCGGTTATGTTAACGGTTAGAG	SOD5 + 1kb flank R
OMM1 (BamHI)	CGGGATCCGATGAATGGTAAGTTAGATTGATAT	Diverge SOD5 F
OMM2 (BamHI)	CGGGATCCGAAAAATAAAATAGATGAGCCATTT	Diverge SOD5 R
OMM3 (XbaI)	GCTCTAGAAAAGAAAAATAAAATAGATGAGCC	Diverge SOD5 R
OMM4(XbaI)	GCTCTAGAGGTACCTGGAGGATGAGGAG	CaHIS1 F
CB20R(BamHI)	CGGGATCCAATATTTATGAGAAACTATCACTTC	CaHIS1 R
OMM8	GGAGCAGTAGAAGCCATACTA	SOD5 R Northern
OMM9	ATGGGCGAGTCCTACAAAACT	SOD5 F Northern
OMM10	ACCAGTGAATCATTTGAAGTT	SOD4 F Northern
OMM11	AGAAGCACTAGTTGATGAACC	SOD4 R Northern
OMM14	TCCAGAAGATGATGAAAGACATG	SOD1 F Northern
OMM15	GTCTAGCACCAGCATGACCAG	SOD1 R Northern
OMM16	AACACAACTTGCTTTGAGTTGAAA	SOD6 F Northern
OMM17	ACAATCACTAGAGATTCCGTGGAT	SOD6 R Northern
OMM18(BamHI)	CGGGATCCCGGTTATGTTAACGGTTAGAG	SOD5 + 1kb flank R
OMM19 (XbaI)	GCTCTAGAAAACCTGGTCTACCAGAAGTT	SOD5 + 1kb flank F
UO106	AAGCCGGTTTTGCCGGTG	ACT1 probe F
UO107	TGGTGAACAATGGATGGAC	ACT1 probe R