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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Biochim Biophys Acta. 2011 Dec 29;1818(3):814–820. doi: 10.1016/j.bbamem.2011.12.020

Figure 4.

Figure 4

EmrE was successfully reconstituted with a wide range of long-chain lipid compositions varying in saturation and head-group. (1H, 15N)-TROSY HSQC spectra of TPP+-bound EmrE reconstituted into isotropic bicelles with the following long-chain lipid compositions: A) DPPC, B) POPC, C) DOPC, D) E. coli polar lipid extract, E) 70% POPE, 20% POPG, 10% POPC, and F) 70% POPC, 20% POPE, 10% POPG. The DPPC spectrum was collected on 2H/15N-EmrE, while all others were collected on 1H/15N-EmrE. Spectra were collected under the same conditions as above.

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