Table 1.
Table of hydrodynamic radii of isotropic bicelles determined via DLS and rotational diffusion NMR.
| Sample | Hydrodynamic radius (nm) | ||||
|---|---|---|---|---|---|
| Water Parametersa | Bicelle Parametersb | ||||
| DLS, 10°C | DLS, 25°C | DLS, 10°C | DLS, 25°C | NMR, 45°C c |
|
| Reconstitution via liposomes, 15N EmrE in DMPC/DHPC bicelles |
3.5 ± 0.8 | 3.0 ± 0.4 | 2.0 ± 0.3 | 2.4 ± 0.3 | 3.2 ± 0.3 |
| Reconsitution via detergent exchange, 15N EmrE in DMPC/DHPC bicelles |
3.2 ± 0.8 | 3.0 ± 0.8 | 1.9 ± 0.4 | 2.4 ± 0.6 | 2.9 ± 0.3 |
| Reconstitution via liposomes, 2H/15N/13C EmrE in DLPC/DHPC bicelles |
3.6 ± 0.8 | 3.3 ± 0.5 | 2.2 ± 0.5 | 2.6 ± 0.4 | 2.7 ± 0.3 |
a
RH values determined from DLS, using the viscosity and refractive index of water.
b
RH values determined from DLS, using the viscosity and refractive index of the isotropic bicelle solution at the given temperature (see methods).
c
Calculated from rotational correlation time using equation 1.