Figure 2.

Characterization of the intracellular distribution of p22. BHK (A-F) or BHK-ER (G-O) cells were fixed in 4% paraformaldehyde and processed for immunofluorescence by using antibodies against p22 (B, E, H, K, and N), tubulin (A), and GM130 (D). BHK-ER cells were used for ER staining (G, J, and M). J-L represent magnifications of the regions of interest (square) shown in G-I. In M-O, BHK-ER cells were treated with 5 μg/ml nocodazole for 1 h before cell fixation. Arrows in D-F indicate colocalization between p22 and GM130 structures. Arrowheads in J-O indicate colocalization between p22 and ER structures. Bars, 10 μm.