Figure 5.

Effect of bulk microinjection of anti-p22 APpep2 antibodies on the organization of the microtubule cytoskeleton. BHK cells were bulk microinjected with APpep2 antibodies (APpep2: A-F and M-O) or APpep2 previously preincubated with pep2 peptide (pep2-competition: G-L) and then allowed to recover at 37°C for 2 h (A-L, 2 h postdig) or 4 h (M-O, 4 h postdig) before fixation. Cells were processed for immunofluorescence by using tubulin antibodies (B, E, H, K, and N). A, D, G, J, and M show APpep2 staining, indicating bulk-microinjected cells. C, F, I, L, and O represent merged images. D-F and J-L are magnifications of the regions of interest shown in A-C and G-I, respectively. Bars, 10 μm.