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Effect of bulk microinjection of myr-p22 or myr-p22-E134A on the organization of the microtubule cytoskeleton. BHK cells were mock (A-C), myr-p22 (D-F and J-L) or myr-p22-E134A (G-I) bulk microinjected. After 2 h at 37°C, cells were fixed and processed for immunofluorescence by using APpep2 (A, D, and G) and tubulin (B, E, and H) antibodies. C, F, and I represent merged images. Bars, 10 μm. Arrowheads in D-F indicate p22-positive microtubule bundles.
