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. Author manuscript; available in PMC: 2012 Aug 3.
Published in final edited form as: Cell. 2012 Feb 3;148(3):502–514. doi: 10.1016/j.cell.2012.01.007

Figure 1. Cortical dynein captures dynamic MT ends.

Figure 1

(A) End-on MT-dynein contacts during spindle positioning in C. elegans embryos. (B) Schematic view of the reconstituted interaction between dynamic MT ends and ‘cortical’ dynein. (C,D) Spinning disc confocal fluorescence images of MTs interacting with a barrier without (C; Movie S1) and with (D; Movie S2) dynein (using the multilayer). Yellow line indicates the position of the barrier. Scale bar indicates 5 μm. (E,F) MTs growing against barriers coated with 100% dynein without (E; Movie S3) and with (F) use of the multilayer. Scale bars indicate 10 μm. (G) Numbers of straight (stalled) and buckled (growing) MTs observed, and numbers of observed release and catastrophe events for various dynein densities (0% refers to no dynein, Multi refers to the use of a multilayer as in D). See Figure S1 for details on the detection of buckled MTs. (H) Release (straight MTs) and catastrophe (buckled MTs) frequencies for the various conditions. Error bars give the statistical errors based on the number of observed events. For cases with fewer than 6 events only an upper limit corresponding to a 95% confidence interval is shown.