TABLE 1.
Primary Antibodies Used
| Antigen | Immunizing antigen | Host species | Source/catalog number |
Dilution |
|---|---|---|---|---|
| B-cell CLL/lymphoma 11B (BCL11B; CTIP2) | GST fusion peptide of the N-terminal region of human CTIP2 (amino acids 1–173) | Rat (monoclonal) | Abcam, Inc. (Cambridge, MA) # ab18465 | 1:100 |
| Calbindin 2 (CALB2) (Calretinin) | Recombinant human calretinin-22k | Mouse (monoclonal) | Swant (Bellinzona, Switzerland) # 6B3 | 1:2,000 |
| Choline acetyltransferase (ChAT) | Purified choline acetyltransferase from human placenta | Rabbit (polyclonal) | Millipore (Billerica, MA) # AB143 | 1:500 |
| D1 Dopamine Receptor | Synthetic peptide corresponding to amino acids 230–248 of human D1 dopamine receptor conjugated to KLH | Rabbit (polyclonal) | Sigma-Aldrich (St. Louis, MO) # D6692 | 1:250 |
| Enkephalin | Synthetic methionine enkephalin conjugated to bovine thyroglobulin with glutaraldehyde | Rabbit (polyclonal) | ImmunoStar, Inc. (Hudson, WI) # 20065 | 1:500 |
| Forkhead box P2 (FOXP2) | Synthetic peptide (C)REIEEEPLSEDLE corresponding to amino acids 703–715 of the C-terminus of human FOXP2 | Rabbit (polyclonal) | Abcam, Inc. # ab16046 | 1:150,000 |
| γ-Aminobutyric Acid (GABA) | GABA-BSA | Rabbit (polyclonal) | Sigma-Aldrich # A2052 | 1:2,000 |
| Glial Fibriallary Acidic Protein (GFAP) | Purified glial fibrillary acidic protein from human brain | Guinea pig (polyclonal) | Advanced ImmunoChemical, Inc. (Long Beach, CA) #31223 | 1:500 |
| Giantin | N-terminal region of human giantin corresponding to amino acids 1–469 | Rabbit (polyclonal) | Covance, Inc. (Princeton, NJ) # PRB-114C | 1:1,000 |
| MAP2 | Rat brain microtubule-associated proteins (MAPs) and isotype was then determined | Mouse (monoclonal) | Sigma-Aldrich # M4403 | 1:1,000 |
| μ opioid receptor | Synthetic peptide corresponding to amino acids 384–398 of rat MOR1 | Rabbit (polyclonal) | ImmunoStar, Inc. # 24216 | 1:250 |
| Nitric oxide synthase 1, neuronal (NOS1; nNOS) | Synthetic peptide corresponding to amino acids 134–148 of human nNOS conjugated to KLH | Rabbit (polyclonal) | ImmunoStar, Inc. # 24431 | 1:500 |
| Parvalbumin | Purified parvalbumin from carp muscle | Mouse (monoclonal) | Swant # 235 | 1:500 |
| Rab5 | Synthetic peptide PKNEPQNPGANSARGR corresponding to amino acids 182–197 of human Rab5 conjugated to KLH | Rabbit (polyclonal) | Abcam, Inc. # ab13253 | 1:1,000 |
| Rab11 | Synthetic peptide corresponding to amino acids 130 –216 of human Rab11 | Rabbit (polyclonal) | Santa Cruz Biotechnology (Santa Cruz, CA) # sc-9020 | 1:100 |
| Slitrk1 | NS0-derived rhSLITRK1 extracellular domain (amino acids 16–616) | Goat (polyclonal) | R&D Systems (Minneapolis, MN) # AF3009 | 1:200 (IF) 1:1,000 (WB) |
| SMI-32 | Homogenized rat hypothalamii; subsequently determined to recognize non-phosphorelated epitopes on the neurofilament heavy chain | Mouse (monoclonal) | Covance, Inc. # SMI-32R | 1:1,000 |
| Substance P | Substance P conjugateded to KLH with carbodiimide | Rabbit (polyclonal) | ImmunoStar, Inc. # 20064 | 1:500 |