Table 3.

SSR primer pairs used for DNA amplification in this study with their parameters of genetic diversity of overall weedy rice (Oryza sativa f. spontanea) populations from Liaoning Province

Primer code	LO	SSR motif	Forward (5′−3′)	Reverse (5′−3′)	Na	He	Molecular weight (bp)
RM11	7	(GA)17	tctcctcttcccccgatc	atagcgggcgaggcttag	7	0·414	121−147
RM14	1	(GA)17	ccgaggagaggagttcgac	gtgccaatttcctcgaaaaa	3	0·016	174−191
RM17	12	(GA)21	tgccctgttattttcttctctc	ggtgatcctttcccatttca	4	0·357	162−187
RM19	12	(ATC)10	caaaaacagagcagatgac	ctcaagatggacgccaaga	1	0·000	216
RM21	11	(GA)21	acagtattccgtaggcacgg	gctccatgagggtggtagag	10	0·494	132−160
RM44	8	(GA)16	acgggcaatccgaacaacc	tcgggaaaacctaccctacc	9	0·713	100−140
RM55	3	(GA)17	ccgtcgccgtagtagagaag	tcccggttattttaaggcg	5	0·056	219−238
RM84	1	(TCT)10	taagggtccatccacaagatg	ttgcaaatgcagctagagtac	4	0·452	110−128
RM167	11	GGAA(GA)16GGGG	gatccagcgtgaggaacacgt	agtccgaccacaaggtgcgttgtc	4	0·549	124−150
RM180	7	(ATT)10	ctacatcggcttaggtgtagcaacacg	acttgctctacttgtggtgagggactg	2	0·492	110−111
RM205	9	(GA)25	ctggttctgtatgggagcag	ctggcccttcacgtttcagtg	2	0·114	154−156
RM211	2	(GA)18	ccgatctcatcaaccaactg	cttcacgaggatctcaaagg	2	0·003	143−156
RM212	1	(GA)24	ccactttcagctactaccag	cacccatttgtctctcattatg	3	0·010	118−136
RM215	9	(GA)16	caaaatggagcagcaagagc	tgagcacctccttctctgtag	4	0·342	146−152
RM219	9	(GA)17	cgtcggatgatgtaaagcct	catatcggcattcgcctg	7	0·723	192−214
RM230	8	(GA)13	gccagaccgtggatgttc	caccgcagtcacttttcaag	3	0·385	253−257
RM253	6	(GA)25	tccttcaagagtgcaaaacc	gcattgtcatgtcgaagcc	7	0·294	133−146
RM276	6	(AG)8A3(GA)33	ctcaacgttgacacctcgtg	tcctccatcgagcagtatca	6	0·458	75−144
RM280	4	(GA)16	acacgatccactttgcgc	tgtgtcttgagcagccagg	4	0·009	151−175
RM289	5	G11(GA)16	ttccatggcacacaagcc	ctgtgcacgaacttccaaag	4	0·375	85−110

LO, location on chromosome number; N_a, observed number of alleles; H_e, expected heterozygosity.