Table 1.
Components of culture media used in the present study
| Elements | g L−1 |
|---|---|
| (A) MK medium (modified from Kano, 1965)* | |
| Hyponex (N-P-K = 6·5–6–19) | 3 |
| Peptone | 2 |
| Sucrose | 30 |
| pH = 5·2 | |
| Elements | mg L−1 |
|---|---|
| (B) ND medium (Tokuhara and Mii, 1993) | |
| NH4NO3 | 480 |
| KNO3 | 200 |
| Ca(NO3)2.4H2O | 470 |
| KCl | 150 |
| MgSO4.7H2O | 250 |
| KH2PO3 | 550 |
| MnSO4.4H2O | 3 |
| ZnSO4.7H2O | 0·5 |
| H3BO3 | 0·5 |
| CuSO4.5H2O | 0·025 |
| Na2MoO4.2H2O | 0·025 |
| CoCl2.6H2O | 0·025 |
| Concentrated H2SO4 | 0·5 μL L−1 |
| myo-Inositol | 100 |
| Nicotinic acid | 1 |
| l-cysteine | 1 |
| Thiamine hydrochloride | 1 |
| Pyridoxine hydrochloride | 1 |
| Adenine | 1 |
| Calcium pantothenate | 1 |
| (+)-Biotin | 0·1 |
| Fe-EDTA | 21 |
| Sucrose | 20 g L−1 |
| pH = 5·4 | |
These culture media were solidified with 2 g L−1 Gerlite®.
* The original Kano medium (Kano, 1965) contained 35 g L−1 sucrose and was solidified with 15 g L−1 agar.