FIG. 4.

PPP1R3G overexpression accelerates postprandial blood glucose clearance in the mouse. A: Overexpression of PPP1R3G in the mouse liver. Male C57BL/6 J mice at 8 weeks of age were infected with adenovirus Ad-GFP or Ad-PPP1R3G (containing a Flag tag at N-terminus) via tail-vein injection. At 9 days post-infection, animals were killed in the fed state. The livers were used in immunoblotting with antibodies as indicated. B: PPP1R3G mRNA level in the liver was measured by RT-qPCR (n = 6 mice/group) from the samples in A. Data are shown as mean ± SD. ***P < 0.001 between the two groups. C: Liver glycogen content from samples in A were measured (n = 8 for Ad-GFP and n = 9 for Ad-PPP1R3G). ***P < 0.001 as compared between the two groups. D–F: Glucose tolerance test (for D) and insulin tolerance test (for F) were performed at 4 days post-infection (n = 7 for Ad-GFP and n = 8 for Ad-PPP1R3G). Mice were fasted for 4 h before glucose injection or insulin injection. Bar graph (E) represents the AUC calculated from the glucose tolerance test. Data are shown as mean ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001 between the two groups of mice.