FIG. 3.

PEDF’s metabolic effects do not depend on central actions. A: The hypothalamus of C57Bl/6 mice was excised, and 18S, PEDF, and ATGL mRNA expression were assessed by qRT-PCR (n = 8). B: C57Bl/6 mice were injected with 0.9 μg PEDF or aCSF and placed in a metabolic monitoring station for assessment of oxygen uptake (VO₂). C: RER was also assessed (n = 8 for PEDF and n = 11 for vehicle). D: Mice were injected with 0.9 μg PEDF or aCSF, and the hypothalami were excised after 6 h to assess mRNA of hypothalamic neuropeptides that modulate feeding and energy metabolism. Pro-opiomelanocortin (POMC), agouti-related peptide (AgRP), neuropeptide Y (NPY), and cocaine- and amphetamine-regulated transcript (CART) were measured (n = 8 per group). All data are presented as means ± SEM.