FIG. 4.

PEDF modulates fatty acid (FA) metabolism in skeletal muscle. L6 myotubes were treated with 100 nmol/L PEDF or saline (vehicle) for 2 h. Total FA uptake (A), incorporation of FA into triacylglycerol (TAG; B) or diacylglycerol (DAG; C), and FA oxidation (D) were assessed using 0.5 mmol/L [1-¹⁴C] palmitate (n = 6–12 for each group). E: Myotube TAG content is shown after 6-h treatment with 0.5 mmol/L oleate in the incubation media (n = 4–6 per group). All data are presented as means ± SEM. *P < 0.05 vs. vehicle. F: PEDF (100 nmol/L) reduces FA oxidation in intact skeletal muscle. Soleus muscles were removed from ATGL^−/− and Wt littermates before assessment of FA oxidation using 0.5 mmol/L [1-¹⁴C] oleate ex vivo. *P < 0.05 vs. vehicle within the same genotype (n = 6 per group). G: Genes associated with FA oxidation and mitochondrial biogenesis (left) and TAG storage and degradation (right) were assessed in vastus lateralis of mice treated with saline or PEDF for 5 days (n = 6 for each group). *P < 0.05 vs. vehicle.