TABLE 1.
Effect of PEDF on muscle lipids in Wt and ATGL−/− mice
| Lipid type | Wt |
ATGL−/− |
||
|---|---|---|---|---|
| Vehicle | PEDF | Vehicle | PEDF | |
| Triacylglycerol | 202,356 ± 50,054 | 72,565 ± 8,131* | 243,282 ± 17,744 | 210,888 ± 52,728 |
| Diacylglycerol | 53,860 ± 4,521 | 42,232 ± 2,304 | 75,273 ± 26,727 | 54,336 ± 24,595 |
| Phosphatidylglycerol | 1,597 ± 181 | 2,052 ± 77 | 1,789 ± 75 | 1,873 ± 101 |
| Phosphatidylinositol | 20,493 ± 4,134 | 22,446 ± 2,594 | 21,346 ± 2,273 | 20,742 ± 1,775 |
| Phosphatidylethanolamine | 34,203 ± 3,803 | 40,704 ± 1,193 | 37,618 ± 952 | 38,991 ± 1,299 |
| Phosphatidylserine | 8,156 ± 463 | 7,630 ± 413 | 8,749 ± 992 | 7,744 ± 385 |
| Cholesterol ester | 3,738 ± 1,348 | 4,606 ± 1,008 | 4,670 ± 1,113 | 3,928 ± 620 |
| Sphingomyelin | 261,548 ± 3,995 | 305,516 ± 15,202† | 355,098 ± 22,736 | 320,042 ± 14,868 |
| Ceramide | 1,924 ± 123 | 1,850 ± 111 | 1,874 ± 147 | 1,809 ± 60 |
| Dihydroceramide | 253 ± 18 | 372 ± 75 | 302 ± 35 | 266 ± 44 |
| Dihexosylceramide | 205 ± 45 | 209 ± 42 | 250 ± 24 | 208 ± 24 |
| Monohexosylceramide | 797 ± 118 | 635 ± 96 | 893 ± 216 | 624 ± 57 |
| GM3 ganglioside | 152 ± 7 | 120 ± 12 | 135 ± 13 | 156 ± 13 |
| Lysophosphatidylcholine | 5,212 ± 1,897 | 3,110 ± 303 | 2,907 ± 320 | 3,135 ± 277 |
Units are expressed as pmol/μmol phosphatidylcholine. Wt, Wt + PEDF, ATGL−/− n = 4; ATGL−/− + PEDF n = 8. Values are mean ± SD.
*P < 0.05 vs. ATGL−/− PEDF.
†P < 0.05 vs. vehicle of the same genotype.