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Expansion of spindle cells on coated Matrigel by serial passages. Single cells derived from collagenase-isolated limbal clusters were seeded at 1 × 105/cm2 on coated Matrigel in MESCM. Spindle cells emerged among small round cells in P0, rapidly proliferated, and became dominant after P2. These spindle cells in P4 could still form spheres when reseeded in 3D Matrigel at a density of 5 × 104/cm2. Scale bar, 100 μm.
