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The average 1H NMR spectrum of human meibum from donors with MGD. The 1H NMR spectra of human meibum may be divided into five regions: The CH2 and CH3 region from 0 to 1.4 ppm, the CH and deshielded CH2 region from 1.4 to 3.9 ppm, the ester region from 3.9 to 4.7, the C═C region from 4.7 to 7 ppm, and the oxidative products region above 7 ppm. The insets at the bottom are presented to show more detail.
