Table 4.

Integrated Band Areas Relative to the Area of the 1.39 CH₂ Resonance

Chemical Shifts (ppm)	Normal Child-Adult Unless Indicated	Meibomian Gland Dysfunction	P	Tentative Assignment†
0.72	0.00151 ± 0.00022	0.00187 ± 0.00025	0.33	No Assignment
0.89	0.0228 ± 0.0033 (adolescent-adult)	0.026 ± 0.003	0.43	─CH3, except n-3
0.94	0.0043 ± 0.0013	0.0038 ± 0.0004	0.78	─CH3, n-3
1.03	0.0014 ± 0.0002	0.0019 ± 0.0003	0.21	─CH2OHCH2 CH3 ─CH2─CH─(CH3)2, isobranched
1.26	0.0041 ± 0.0002	0.0029 ± 0.0002	>0.0001*	─COO(CH2)nCH3, short chain esters
1.29	0.058 ± 0.009	0.062 ± 0.007	0.72	─COO(CH2)nCH3, short chain esters
1.39	1	1	1	─CH2)n─, core,
1.70	0.43 ± 0.043	0.52 ± 0.005	0.17	R─CH2─CH2─COO─R
4.10	0.026 ± 0.0034	0.025 ± 0.003	0.83	ROCH2-alkyl, wax ester
4.18	0.0031 ± 0.0005	0.003 ± 0.001	1	CHCH2OR; C1, 3 protons, glyceryl. 4 H per molecule
4.25	0.0036 ± 0.0004	0.0033 ± 0.0003	0.55	C1, 3 protons, glyceryl
4.60	0.0047 ± 0.0008	0.0036 ± 0.0007	0.31	R─O─CH, C3 of cholesterol ester
4.85	0.014 ± 0.0044 (adolescent-adult)	0.0121 ± 0.0038	0.76	═CH─, terpenoid
5.00	0.050 ± 0.097 (adolescent-adult)	0.032 ± 0.0047	0.063	═CH─, terpenoid
5.20	0.036 ± 0.0077 (adolescent-adult)	0.0175 ± 0.0029	0.008*	═CH─, terpenoid
5.45	0.206 ± 0.013	0.212 ± 0.016	0.79	─CH═CH─
7.90	0.0088 ± 0.0013	0.0066 ± 0.0004	0.024*	Hydroperoxides
8.70	0.108 ± 0.012	0.024 ± 0.006	>0.001*	Hydroperoxides
9.65	0.002 ± 0.0008	0.0053 ± 0.0036	0.6	trans-2-Alkenals
9.71	0.00026 ± 0.00004	0.00043 ± 0.00007	0.96	R─CH═O, n-alkenals

^*Significantly different, P < 0.05.

^†The bold underlined proton is the proton for which the tentative assignment was made.