Figure 3.

Analysis of myelin loss in optic nerves of RTL342M- and vehicle-treated HLA-DR2 mice. (A–C) Representative images of long optic nerve sections at ON collected on days 16, 30, and 62 PI stained with Luxol fast blue to label myelin. (A) Normal optic nerve shows compact myelin staining; longitudinal sections of optic nerves from (B) vehicle- and (C) RTL342M-treated mice collected at different times after immunization with MOG. (D) Evaluation of demyelination of optic nerves by quantification of the intensity of the staining and adjusted to 0.1 mm² showed significantly greater demyelination in vehicle-treated mice. Values are expressed as the mean ± SEM of staining intensity from four optic nerves per group.