Figure 5.

Oligodendrocyte immunofluorescent labeling of the vehicle- and RTL342M-treated optic nerves. (A) Double-immunofluorescent labeling in the optic nerves collected at 30 and 62 PI with anti-MOG antibodies as a marker for oligodendrocytes and with anti-RT97 for labeling axons: anti-MOG (red), anti-RT97 (green) antibodies and colocalization (yellow). (A, B, white arrows) Red-labeled MOG⁺ myelin-producing cells. (B) Double immunofluorescent labeling of 62PI vehicle and RTL-treated optic nerves with anti-MOG (red) and anti-CD11b antibodies for labeling of mϕ/microglia (green); yellow arrows: microglia, nuclear labeling with DAPI (blue).