Skip to main content
. 2012 Jan 25;53(1):448–453. doi: 10.1167/iovs.11-8999

Figure 2.

Figure 2.

(A) Complementation of PA14ΔexoU with pUCPexoU returned bacterial traversal of human corneal epithelial cells to wild-type PA14 levels. Complementation of PA14ΔexoU with pUCPexoUD344A, which lacks phospholipase activity, did not promote traversal and was similar to the empty vector (pUCP18) control, except at 2 hours. All strains grew similarly in the apical compartment. (B) Complementation of PA14ΔexoU with pUCPexoU caused TER disruption similar to wild-type. Complementation with phospholipase inactive exoU (pUCPexoUD344A) did not affect TER. (C) Complementation of PA14ΔexoU with pUCPexoU also restored cytotoxicity toward multilayered human corneal epithelial cells after 3 hours, as shown by trypan blue staining. Top (left to right): no bacteria control, PA14ΔexoU, and PA14ΔexoU complemented with empty vector pUCP18. Bottom (left to right): PA14 wild-type, PA14ΔexoU complemented with pUCPexoU and PA14ΔexoU complemented with pUCPexoUD344A (phospholipase domain mutant).