Figure 6.

ADAMTSL4 accelerates fibrillin-1 deposition in the matrix of cultured fibroblasts. Fetal bovine nuchal ligament cells (fBNL) cells were cultured to confluence in media that had previously been conditioned by HEK293F cells stably transfected with ADAMTSL4 expression plasmid or empty vector as indicated. After 4 days of culture, cells were fixed and stained for fibrillin-1 (green), and nuclei were stained with DAPI (blue). Three representative fields (20× magnification) from independent experiments are shown for both vector-conditioned and ADAMTSL4-conditioned medium–treated fBNL cells. Note the enhanced fibrillin-1 fluorescence in cells cultured in the presence of exogenous ADAMTSL4. Scale bars, 50 μm.