Figure 3.

(A) A representative FISH image (founder 53-1) indicating the chromosomal integration site of the human RHO transgene. All founder pigs demonstrated a single integration site, except 51-1 for which a signal was not detected. (B) Southern blot of six living founders. A total of 10 μg digested with NsiI was loaded per lane. Pig 18-1 was the original source of the fetal fibroblasts used to create the transgenic founders. Signal for all transgenic pigs was detected, while 51-1 and 64-1 demonstrated the least signal. Pigs 53-1 and 63-2 most likely had a greater number of human RHO copies integrated into the genome.