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. 2011 Oct 14;18(3):129–135. doi: 10.1093/molehr/gar065

Figure 3.

Figure 3

Subcellular location of LHR variant in 293T cells. (A and  H) LHR-WT was subcloned into pEGFPN2 and the resulting plasmid was transiently transfected into 293T cells. The expressed LHR-WT protein was localized to the plasma membrane. (B) LHR-Del7 was subcloned and expressed as described for WT; the expressed mutant protein was mainly localized to the plasma membrane. (C) Western blot showed the expression of LHR-Del7 and LHR-WT with anti-FLAG and anti-GFP antibody respectively. (D–F) 293T cells were co-transfected with pGFPN2-LHR-WT and pCDNA3.1-LHR-Del7 at different ratios: 1:1 (D), 1:2 (E) and 1:5. (F) LHR-WT localized to the plasma membrane in all cases. (G) Immunofluorescence analysis detected cell surface expression of LHR-Del7 with anti-FLAG antibody. (I) Co-localization of LHR-Del7 and LHR-WT in the cell membrane (A, B, D, E, F, G, H, I; ×400).