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(A) Sum of ensemble average distortion and van der Waals interaction energies. The more negative energies are more stabilizing. (B) ΔTm = Tm (modified) − Tm (unmodified), the differences between the thermal melting temperatures (Tm) of the modified and unmodified duplexes. Data is given in Table 2. The ΔTm values are derived from experiments with identical concentrations of modified or unmodified DNA duplexes (Table 2).
