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. 2012 Feb 23;7:1101–1114. doi: 10.2147/IJN.S28753

Figure 9.

Figure 9

(A) Phase contrast micrographs of rabbit corneal keratocyte cultures incubated for 2 days at 37°C with extract medium conditioned with various chondroitin-4-sulfate-modified porous gelatin scaffolds (control, GCS000, GCS005, GCS025, and GCS125); (B) gene expression of interleukin-6 (IL-6) in rabbit corneal keratocytes incubated with extract medium conditioned with various chondroitin-4-sulfate-modified porous gelatin scaffolds for 2 days, measured by real-time reverse transcription polymerase chain reaction.

Notes: Scale bars, 100 μm; glyceraldehyde-3-phosphate dehydrogenase was used for normalization; data in the experimental groups are percentages relative to that of control groups (without materials); values are mean plus or minus standard deviation (n = 3); scaffold groups labeled according to chondroitin-4-sulfate concentration used (0%, 0.05%, 0.25%, or 1.25% (w/v)): GCS000, GCS005, GCS025, and GCS125.

Abbreviation: mRNA, messenger RNA.