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. 2012 Jan 10;5:18. doi: 10.1186/1756-0500-5-18

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Copyright ©2011 Petit et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Average expression stability values (M) of seven candidate reference genes computed using geNorm. Ranking was performed for all RNA samples (TOTAL), and for the subsets +2.5H, +6H and RESISTANT. M-values of the remaining genes at each step during stepwise exclusion of the least stable gene are shown. The genes are ranked according to increasing expression stability (decreasing M-values) starting from the least stable gene on the left. The two most stable genes are on the right. The horizontal dotted line indicates the cutoff M-value (1.5).