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. 2012 Jan 10;5:18. doi: 10.1186/1756-0500-5-18

Table 3.

Ranking of the candidate reference genes according to their stability value using BestKeeper and NormFinder

	BestKeeper					NormFinder

Reference genes^a	All samples				All samples		Herbicide effect		Phenotype effect

							BEFORE/+2.5H+6H		SENSITIVE/RESISTANT

	SD^b	r ^c	Ranking order		SV^d	Ranking order	SV	Ranking order	SV	Ranking order

26S	0.46	0.80*	1		0.335	1	0.148	1	0.132	1

*TUB*	1.07	0.88*	2		0.488	2	0.204	4	0.156	2

*GAPDH*	0.93	0.70*	3		0.494	3	0.188	3	0.242	4

*UBQ*	0.81	0.67	4		0.515	4	0.175	2	0.254	5

25S	0.50	0.53	5		0.669	5	0.258	5	0.279	6

18S	0.96	0.42	6		1.039	7	0.378	7	0.370	7

EF1	1.45	0.85*	7		0.813	6	0.332	6	0.231	3

^aThe three reference genes selected are in bold

^bStandard deviation of Cq values. SD values higher than the cut-off value (1.00) are underlined

^cPearson coefficient of correlation

^dStability value

*: associated p value <0.001