Table 2.
Multi-centre evaluation of Speed-oligo Mycobacteria and comparison to Genotype CM
| Mycobacterium species a | no. of Strains | Speed-oligo results | Genotype CM results d | |
|---|---|---|---|---|
| Bandsb | Identificationc | |||
| IML Gauting, Germany | 59 | |||
| M. tuberculosise | 13 | (3), 4, 7 | M. tuberculosis complex | M. tuberculosis complex |
| M. avium | 14 | 5, 7 | MAIS group | M. avium |
| M. intracellulare | 9 | 5, 7 | MAIS group | M. intracellulare |
| M. intracellulare | 1 | 5, 7 | MAIS group | Mycobacterium spp. |
| M. kansasii | 7 | 3, 7 | M. kansasii | M. kansasii |
| M. kansasii | 1 | 3, 7 | M. kansasii | Mycobacterium spp. |
| M. abscessus | 1 | 1, 7 | M. chelonae/M. abscessus complex | M. abscessus |
| M. chelonae | 1 | 1, 7 | M. chelonae/M. abscessus complex | M. chelonae |
| M. gordonae | 7 | 2, 7 | M. gordonae | M. gordonae |
| M. fortuitum | 2 | 6, 7 | M. fortuitum | M. fortuitum |
| M. peregrinum | 1 | (6), 7 | M. fortuitum | M. peregrinum |
| M. xenopi | 1 | 7 | Mycobacterium spp. | M. xenopi |
| Tsukamurella pulmonis | 1 | - | no Mycobacterium spp. | Gram+ bacteria, high G+C content |
| Bioscientia Ingelheim, Germany | 60 | |||
| M. tuberculosise | 13 | (3), 4, 7 | M. tuberculosis complex | M. tuberculosis complex |
| M. bovis BCGe | 1 | (3), 4, 7 | M. tuberculosis complex | M. tuberculosis complex |
| M. avium | 4 | 5, 7 | MAIS group | M. avium |
| M. intracellulare | 6 | 5, 7 | MAIS group | M. intracellulare |
| M. scrofulaceum | 1 | 5, 7 | MAIS group | M. scrofulaceum |
| M. kansasii | 1 | 3, 7 | M. kansasii | M. kansasii |
| M. chelonae | 3 | 1, 7 | M. chelonae/M. abscessus complex | M. chelonae |
| M. gordonae | 14 | 2, 7 | M. gordonae | M. gordonae |
| M. fortuitum | 2 | 6, 7 | M. fortuitum | M. fortuitum |
| M. celatum | 4 | 7 | Mycobacterium spp. | Mycobacterium spp. |
| M. flavescens | 1 | 7 | Mycobacterium spp. | Mycobacterium spp. |
| M. malmoense | 1 | 7 | Mycobacterium spp | M. malmoense |
| M. montefiorense | 1 | 7 | Mycobacterium spp. | Mycobacterium spp. |
| M. peregrinum | 1 | 6, 7 | M. fortuitum | M. peregrinum |
| M. simiae | 3 | 7 | Mycobacterium spp. | Mycobacterium spp. |
| M. xenopi | 2 | 7 | Mycobacterium spp. | M. xenopi |
| Nocardia spp. | 2 | - | no Mycobacterium spp. | n.d. |
| KLINLAB Prague, Czech Rep. | 75 | |||
| M. tuberculosise | 26 | (3), 4, 7 | M. tuberculosis complex | M. tuberculosis complex |
| M. bovis BCGe | 1 | (3), 4, 7 | M. tuberculosis complex | M. tuberculosis complex |
| M. avium | 13 | 5, 7 | MAIS group | M. avium |
| M. intracellulare | 5 | 5, 7 | MAIS group | M. intracellulare |
| M. kansasii | 3 | 3, 7 | M. kansasii | M. kansasii |
| M. abscessus | 1 | 1, 7 | M. chelonae/M. abscessus complex | M. abscessus |
| M. chelonae | 1 | 1, 7 | M. chelonae/M. abscessus complex | M. chelonae |
| M. gordonae | 9 | 2, 7 | M. gordonae | M. gordonae |
| M. fortuitum | 9 | 6, 7 | M. fortuitum | M. fortuitum |
| M. lentiflavum | 2 | 7 | Mycobacterium spp. | Mycobacterium spp. |
| M. marinum | 1 | 3, 7 | M. kansasii | M. marinum |
| M. xenopi | 4 | 7 | Mycobacterium spp. | M. xenopi |
| Khartoum, Sudan | 48 | |||
| M. tuberculosise | 16 | (3), 4, 7 | M. tuberculosis complex | M. tuberculosis complex |
| M. tuberculosise + M. fortuitum | 7 | (3), 4, 6, 7 | M. tuberculosis complex + M. fortuitum | M. tuberculosis complex + M. fortuitum |
| M. fortuitum | 25 | 6, 7 | M. fortuitum | M. fortuitum |
| TOTAL | 242 | |||
aDifferentiation to species level by use of Genotype CM, Genotype AS, Genotype MTBC or sequencing (16S rDNA or hsp65 gene).
bNumbers in brackets indicate weaker staining.
cMAIS group: M. avium M. intracellulare M. scrofulaceum group.
dIdentification results by Genotype CM are shown. n.d.: not determined.
eAll MTBC isolates yielded weak TL3 signals in addition to TL4