Skip to main content
. 2011 Dec 20;11:523. doi: 10.1186/1471-2407-11-523

Figure 2.

Figure 2

Re-expression of SAV1 inhibits cell proliferation and colony formation. a SAV1 protein is expressed in 786-O and 769-P cells transduced with SAV1. Total cell lysates were subjected to Western blot analysis using anti-SAV1 antibody and anti-GAPDH antibody, respectively. SAV1 protein was detected in SAV1-transduced 786-O and 769-P cells (SAV1), but not in cells transduced with pLenti7.3/V5 empty vector (control). b Re-expression of SAV1 inhibits colony-formation in 786-O and 769-P cells. Upper column; colony-forming activities of 786-O and 769-P cells transduced with control vector or pLenti-SAV1. Colony numbers were significantly reduced in 786-O and 769-P cells transduced with pLenti-SAV1 (SAV1) compared with those transduced with pLenti7.3/V5 (control). Experiments were performed in triplicate. Lower column; A representative result of colony formation assay using 786-O cells transduced with the control vector and those transduced with SAV1.