Figure 3.

Influence of gelsolin on integrin function. (A) Recruitment of α2-integrin (labeled with PE-conjugated antibody) around bound collagen beads was similar in Gsn^- and WT cells, suggesting that integrin avidity was not affected by Gelsolin. (B) For assessment of antibody-induced integrin activation, Gsn^- and WT cells were incubated with beads at 8:1 (bead:cell ratio) in the presence of β1-integrin activating antibody (9EG7) and analyzed by fluorescence microscopy. There was 50% enhancement in collagen bead binding in Gsn^- cells but no change in WT cells (p < 0.01). Data are mean ± SEs of mean number of beads bound per cell.