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. 2012 Mar 2;7(3):e32707. doi: 10.1371/journal.pone.0032707

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Yue et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The expression of Nanog was analyzed by FACS after 10 passages on MMC-MEFs, six passages on new chemically fixed MEFs, or three passages on reused chemically fixed MEFs. (A) GA-fixed MEFs. (B) FA-fixed MEFs. MEF-new, chemically fixed MEFs used for cell culture for the first time; MEF-R3, chemically fixed MEFs reused for the third time; MEF-R4, chemically fixed MEFs reused for the fourth time.