Figure 1. Age-dependent effect of systemic imipramine on TrkB phosphorylation and signaling in the mouse brain.

(a) Phosphorylation of TrkB phospholipase-Cγ1 (PLCγ1) binding site (Y816) after acute imipramine treatment (30 mg/kg, 30 min, i.p.) in prefrontal cortex (PFC) and hippocampus (HC). Phospho-TrkB values are normalized against total TrkB levels. (b) Phosphorylation of CREB (Ser¹³³) after acute imipramine treatment (30 mg/kg, 30 min, i.p.) in prefrontal cortex (PFC) and hippocampus (HC). Phospho-CREB values are normalized against total CREB levels. (c) The effect of acute imipramine treatment (30 mg/kg, 30 min, i.p.) on the association of phosphorylated PLCγ1 (Tyr⁷⁸³) with catalytic TrkB receptors in P8 mouse pup hippocampus. (d) The effect of acute imipramine treatment (30 mg/kg, 30 min, i.p.) on the association of phosphorylated PLCγ1 (Tyr⁷⁸³) with catalytic TrkB receptors in P25 mouse pup hippocampus. Results are expressed as percentage of respective control. A t-test was performed between each control and treated group of animals at the different ages; *P<0.05, ** P<0.01, *** P<0.001. n = 6–7 per group.