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. 2012 Mar 2;7(3):e32869. doi: 10.1371/journal.pone.0032869

Figure 5. TrkB responsiveness to ex vivo BDNF is not altered by antidepressant drugs or cAMP signalling.

Figure 5

(a) P60 mice were pretreated acutely with imipramine (Imi; 30 mg/kg i.p., 60 min) or saline (Sal), hippocampi were collected, sliced and incubated ex vivo with or without BDNF (50 ng/ml) for 5 minutes at 37°C. TrkB phosphorylation (Y816) was analyzed with western blotting. (b) P24 hippocampal microslices were pre-incubated at 37°C for 15 minutes with vehicle or sp-cAMP (10 µM) and then exposed to BDNF (50 ng/ml) or vehicle at 37°C for another 15 minutes. TrkB phosphorylation (Y705/6) was analyzed with western blotting. (c) Adult animals were chronically treated for 21 days with vehicle (Veh) or fluoxetine (0.08 mg/ml) in drinking water, hippocampi were collected, sliced and incubated ex vivo with or without BDNF (50 ng/ml) for 5 minutes at 37°C. TrkB phosphorylation (Y816) was analyzed with western blotting. Phospho-TrkB values are normalized against total TrkB levels. Two-Way ANOVA followed with Bonferroni post hoc test was performed for statistical analysis; **P<0.01, *** P<0.001. n = 3–6 per group.