Fig. 2. Mg²⁺ is required for gastrulation.

(A) Dorsal injection of XTRPM7 MOs (75 ng) in embryos cultured in 0.1X MMR (0.1 mM Mg²⁺) inhibited gastrulation. Supplementation of Mg²⁺, but not Ca²⁺, into the culture media at 5 mM at stage 10.5 rescued the gastrulation phenotype. (B) Quantification of the effects of Mg²⁺ and Ca²⁺ supplementation on embryos injected with XTRPM7 MOs; the number of embryos is shown above each bar. (C) Embryos injected dorsally with XTRPM7 MOs or TRPM7 RNA had no defects in expression of mesodermal marker genes Chordin, Goosecoid, Xbra and XWnt8 as monitored by RT-PCR analysis; EF1-α was used as a loading control.