Fig. 3. XTRPM7 is required for convergent extension movements.

(A) Dorsal marginal zone (Keller) explant assay revealed that overexpression of TRPM7 (2 ng) and injection of XTRPM7 MOs inhibit convergent extension movements. Dominant negative Dishevelled (Xdd1, 2 ng) was used as a positive control. Inhibition of convergent extension produced by depletion of XTRPM7 was rescued by co-injection of XTRPM7 MOs with TRPM7 RNA (400 pg) and the Mg²⁺ transporter SLC41A2 (400 pg), as well as supplementation of the buffer with 5 mM Mg²⁺ but not 5 mM Ca²⁺. (B) Quantification of Keller explant assay; bars indicate the average length to width ratio of Keller explants and the number of explants analyzed is shown above each bar.