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. Author manuscript; available in PMC: 2012 Mar 2.
Published in final edited form as: Dev Biol. 2010 Dec 9;350(2):348–357. doi: 10.1016/j.ydbio.2010.11.034

Fig. 4. XTRPM7 is required for dorsal mesodermal cell polarization, elongation and alignment.

Fig. 4

(A) Confocal imaging analysis revealed that injection of TRPM7 RNA and XTRPM7 MOs inhibits dorsal mesodermal cell polarization and elongation. Control cells were labelled with EGFP-CAAX (green, 500 pg) and membrane-tethered Cherry (red, 500 pg) was co-injected with either TRPM7 RNA (2 ng), dominant negative Dishevelled (Xdd1) RNA (2 ng), XTRPM7 MOs (75 ng), or with XTRPM7 MOs (75 ng) and TRPM7 RNA (400 pg). (B) Illustration of calculations of average length to width ratio and average angular deviation. Length to width ratio was measured by taking the length of the cell (red arrow) divided by the width of the cell (blue line). Angular orientation of the cell was measured by taking the absolute value of the angle of the red arrow in reference to the lateral axis (L) and was used for calculations of average angular deviation. (C) Injection of Xdd1 RNA and TRPM7 RNA or injection of the XTRPM7 MOs disrupted dorsal mesodermal cell elongation as quantified by the length/width ratio of cells. The error bars represent the standard deviation (s.d.) from at least three independent experiments and the number of cells examined is shown at the top of each bar. (D) Injection of Xdd1 RNA and TRPM7 RNA or injection of the XTRPM7 MOs disrupted alignment of dorsal mesodermal cells undergoing convergent extension. The error bars represent the s.d. from at least three independent experiments and the number of cells examined is shown at the top of each bar.