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. Author manuscript; available in PMC: 2012 Mar 2.
Published in final edited form as: Dev Biol. 2010 Dec 9;350(2):348–357. doi: 10.1016/j.ydbio.2010.11.034

Fig. 6. TRPM7 regulates Rac but not Rho activation levels.

Fig. 6

(A) Western blot analysis and quantification of levels of activated Rac at stage 10.5 during gastrulation. Injection of 75 ng of XTRPM7 MOs increased levels of activated Rac, which could be prevented by co-injection of 500 pg of TRPM7 RNA. (B) Quantification of the results in (A), the error bars represent the s.d. from at least five independent experiments (C) Western blot analysis and quantification of levels of activated Rho at stage 10.5 during gastrulation. Levels of activated Rho were unaffected by injection of XTRPM7 MOs or TRPM7 RNA. (D) Quantification of the results in (C); error bars represent the s.d. from at least four independent experiments