Figure 4.

rabip4′ regulates Tf transport through early endosomes. HeLa cells were transfected with VSV-G-tagged rabip4′ (A-D) or the VSV-G-tagged CC13-FYVE rabip4′ mutant (E-H). Cells were labeled with mouse anti-VSV-G tag followed by Alexa488-goat anti-mouse IgG (A-H). Alexa594-Tf was taken up for 30 min at 16°C, washed, and chased at 37°C for 0 min (A′ and E′), 10 min (B′ and F′), 20 min (C′ and G′), and 40 min (D′ and H′) in the presence of 50 mM Desferal. The corresponding merged images are shown in A″-H″. Bar, 10 μm.