Figure 8.

rab4 and rab5 colocalize with rabip4′. HeLa cells expressing VSV-G-rabip4′ and rab4 (A-C), or VSV-G-rabip4′ and EGFP-rab5 (D-F) were labeled with a mAb against the VSV-G tag and counterstained with either Alexa488-goat anti-mouse IgG (A) or Alexa594-goat anti-mouse (D). rab4 was labeled with a rabbit antibody and stained with Cy3-goat anti-rabbit IgG (B), whereas rab5 was detected by EGFP fluorescence (E). Merged images are shown in C and F, and colocalization is indicated by arrows. Bar, 10 μm.