Figure 7.

In vivoincrease in WBC associated with decreased G-CSF degradation. White blood cell count was higher in mice treated with a mutant G-CSF engineered for decreased degradation via increased dissociation in the endosomal compartment (D113H) than those treated with wildtype G-CSF. Five animals were treated with 5FU to inhibit haematopoiesis 24 h prior to treatment with the colony stimulating factor. “Veh” denotes sham treatment with PBS rather than 5FU. *p < 0.001 versus vehicle-treated controls.