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. 2011 Nov 22;61(4):543–553. doi: 10.1136/gutjnl-2011-301012

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© 2012, Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.

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Cell architecture and inflammation in visceral white adipose tissue (WAT). (A) Adipocyte area and distribution, (B) mean adipocyte area, (C) total cell count of the stroma vascular fraction (SVF)/g visceral WAT, (D) endothelial (CD31+), preadipocytes (CD34+), macrophages (F4/80/CD11b+), total lymphocytes and T cells (CD3+), (E) macrophage immunostaining and number (%) per adipocyte count, (F) western blot analysis of phosphorylated and total proteins involved in inflammatory pathways in mice fed a high-fat diet (HFD) which became diabetic (HFD-D), diabetes-resistant (HFD-DR) and mice fed a diet supplemented with gluco-oligosaccharides (HFD+GOS). Data are shown as mean ± SEM; *p<0.05, **p<0.01, ***p<0.001 (unpaired Student t test; n=5–12 per group).