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. Author manuscript; available in PMC: 2013 Feb 24.
Published in final edited form as: Chem Biol. 2012 Feb 24;19(2):218–227. doi: 10.1016/j.chembiol.2011.12.016

Figure 2. Cholesterol sidechain-derived propionate is incorporated into SL-1 and increases the mass of this lipid.

Figure 2

(A) The cholesterol molecule. Carbon 26 is indicated. (B) Total polar lipids were extracted from [26-14C]-cholesterol-(lane 1) and [1,2-14C]-acetate (lane 2)-fed bacteria, separated by thin layer chromatography, and detected by autoradiography. (C) The major species labeled by cholesterol was isolated and identified as SL-1 using ESI-TOF MS. The m/z peaks depicted match the previously described lipoforms of SL-1 (Kumar et al., 2007). TLC purified samples of SL-1 from cholesterol- or glycerol-fed bacteria were compared to demonstrate that growth on cholesterol increased the mass of SL-1.