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Cellular repair assay to study mutated MutY enzymes. A plasmid vector containing a central OG:A mismatch within a BMTI restriction site is transformed into JM101 muty- E. coli cells expressing a specific MutY enzyme (WT or modified MutY). Restriction fragment and sequence analyses of the resulting plasmids isolated from E. coli indicate the amount of G:C bp at the original lesion site defining the extent of MutY-mediated repair.
